Simultaneous usage of the 2D STED and AFM sample manipulation mode.
The manipulation of individual beads (diameter: 100 nm) with the AFM tip can be watched online with STED superresolution.
For the JPK Instruments Nanowizard 4 specifications please see the following page:
For specifications concerning STED/RESOLFT and confocal imaging please see the respective modules.
B. Harke, J. V. Chacko, H. Haschke, C. Canale, A. Diaspro, " A novel nanoscopic tool by combining AFM with STED microscopy", Optical Nanoscopy 2012, 1:3
J. V. Chacko, B. Harke, C. Canale, A. Diaspro, "Cellular level nanomanipulation using atomic force microscope aided with superresolution imaging", J Biomed Opt. 2014; 19(10):105003.
J. V. Chacko, C. Canale, B. Harke, A. Diaspro, "Sub-diffraction nano manipulation using STED AFM", PLoS One. 2013 Jun 17;8(6):e66608