This xz-slice through mouse inner-ear hair cells using easy3D STED and RESCue STED demonstrates the impressive performance that can be achived by applying both techniques simultaneously. Since standard 3D superresolution places more photoinduced stress on the marker, the key ingredients that made this image possible where 1st: producing a STED-zero point of exceptional quality using easy3D STED, and 2nd: further reducing the light dose in the sample with RESCue STED.
Our patent protected easy3D STED technology produces superior 3D STED data – please check out our new publication:
J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, G. Donnert, "Three dimensional live-cell STED microscopy at increased depth using a water immersion objective", Review of Scientific Instruments (2018)
easy3D DyMIN STED volume imaging of Neurons with unprecedented resolution!
Confocal vs. DyMIN STED imaging:
Confocal and easy3D DyMIN STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to Abberior STAR 635P. Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany. Scale Bar, 2 µm.
DyMIN STED complements our existing list of intelligent STED illumination schemes, namely RESCue STED and MINFIELD STED. All three intelligent illumination schemes for STED dramatically reduce the light irradiation on your sample (DyMIN STED up to two orders of magnitude) and are exclusively available @ Abberior Instruments.
Explanation videos of DyMIN, RESCue and MINFIELD STED:
DyMIN STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017:
J. Heine, M. Reuss, B. Harke, E. D'Este, S. J. Sahl and S. W. Hell, "Adaptive-illumination STED nanoscopy", PNAS (2017)
Nature review on DyMIN STED: G. Pacchioni, "Always look on the bright side of the fluorophore", Nature Review Materials (2017)
Surface Rendering of 2 color easy3D STED volumen imaging of a cleared adult kidney sample of a rat.
Get to know a part of our Abberior Instruments Team!
Follow along with the incredibly quick installation of a STEDYCON super resolution microscope, uncut. We go from opening the carton to the first super-resolution STED image in under three minutes. Don't forget to check out the real time movie here: https://youtu.be/wnFnyt8yJyA
easyCommander is Imspector's brand new graphical user interface for Abberior Instruments Expert Line microscopes.
It largely simplifies your experimental workflow and allows you to acquire STED images with a few clicks.