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Abberior Instruments GmbH
Abberior Instruments GmbH

IMAGING BEYOND BARRIERS!

DyMIN STED + easy3D STED = unprecedented 3D STED resolution

Please check out the new Publication (link below): 

A combination of expansion microscopy and DyMIN 3D STED reveals the ultrastructure of centrioles. Chlamydomonas reinhardtii centrioles immunostained with Abberior STAR RED for polyglutamylated tubulin. Samples by P. Guichard & D. Gambarotto (University of Geneva).

D. Gambarotto, F. U. Zwettler, M. Le Guennec, M. Schmidt-Cernohorska, D. Fortun, S. Borgers, J. Heine, J. G. Schloetel, M. Reuss, M. Unser, E. S. Boyden, M. Sauer, V. Hamel & P. Guichard, “Imaging cellular ultrastructures using expansion microscopy (U-ExM)”, Nature Methods (2019)

DFG supports MINFLUX microscopy from Abberior Instruments

On December 10, 2018, the German Research Foundation (DFG) announced the funding of three high-performance microscopes from Abberior Instruments as part of the major instrumentation initiative "Novel experimental light microscopy for research". A total of 13 instruments from different manufacturers will be funded with a total budget of EUR 14.5 million.

Further Information: Press Release (English & German) 

Correlative microscopy approach for biology using X-ray holography and STED microscopy

Please check out the new Publication (link below): 

(1st row: T. Salditt, M. Bernhardt, S. Köster; 2nd row: M. Osterhoff, H. Mittelstädt, A. Wittmeier)

M. Bernhardt, J.-D. Nicolas, M. Osterhoff, H. Mittelstädt, M. Reuss, B. Harke, A. Wittmeier, M. Sprung, S. Köster, T. Salditt, "Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy", Nature Communications Volume 9, Article number: 3641 (2018)

Three dimensional live-cell STED microscopy at increased depth using a water immersion objective

Our patent protected easy3D STED technology produces superior 3D STED data – please check out our new publication:

Spherical abberations corrected z-STED raw data

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, G. Donnert, "Three dimensional live-cell STED microscopy at increased depth using a water immersion objective", Review of Scientific Instruments (2018)

DynamicPLUS

DyMIN STED

easy3D DyMIN STED volume imaging of Neurons with unprecedented resolution!

Confocal vs. DyMIN STED imaging:

Confocal and easy3D DyMIN STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to Abberior STAR 635P. Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany. Scale Bar, 2 µm.

DyMIN STED complements our existing list of intelligent STED illumination schemes, namely RESCue STED and MINFIELD STED. All three intelligent illumination schemes for STED dramatically reduce the light irradiation on your sample (DyMIN STED up to two orders of magnitude) and are exclusively available @ Abberior Instruments.

Explanation videos of DyMIN, RESCue and MINFIELD STED:

 

DyMIN STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017: 

J. Heine, M. Reuss, B. Harke, E. D'Este, S. J. Sahl and S. W. Hell, "Adaptive-illumination STED nanoscopy", PNAS (2017)

Nature review on DyMIN STED: G. Pacchioni, "Always look on the bright side of the fluorophore", Nature Review Materials (2017)

Easy3D STED volume imaging deep in tissue

Surface Rendering of 2 color easy3D STED volumen imaging of a cleared adult kidney sample of a rat.

The Abberior Instruments Team

Get to know a part of our Abberior Instruments Team!

From zero to STED in no time

Follow along with the incredibly quick installation of a STEDYCON super resolution microscope, uncut. We go from opening the carton to the first super-resolution STED image in under three minutes. Don't forget to check out the real time movie here: https://youtu.be/wnFnyt8yJyA