IMAGING BEYOND BARRIERS!

J.-G. Schloetel, J. Heine, A. F. Cowman, M. Pasternak, "Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites", Nature Scientific Reports, Volume 9, Article number: 4674 (2019)

The recent and most fruitful collaboration between Abberior Instruments and SVI has brought about cutting-edge superresolution at maximum convenience and benefit for our STEDYCON users.

As exemplified in the panels below, the combination of top-notch STED imaging with a sophisticated deconvolution method reveals the faintest details in the sample. Moreover, all this unfolds at the push of a button!

For any questions & quotations: sales@abberior-instruments.com

Please check out the new Publication (link below): 

A combination of expansion microscopy and DyMIN 3D STED reveals the ultrastructure of centrioles. Chlamydomonas reinhardtii centrioles immunostained with Abberior STAR RED for polyglutamylated tubulin. Samples by P. Guichard & D. Gambarotto (University of Geneva).

D. Gambarotto, F. U. Zwettler, M. Le Guennec, M. Schmidt-Cernohorska, D. Fortun, S. Borgers, J. Heine, J. G. Schloetel, M. Reuss, M. Unser, E. S. Boyden, M. Sauer, V. Hamel & P. Guichard, “Imaging cellular ultrastructures using expansion microscopy (U-ExM)”, Nature Methods (2019)

M. Bernhardt, J.-D. Nicolas, M. Osterhoff, H. Mittelstädt, M. Reuss, B. Harke, A. Wittmeier, M. Sprung, S. Köster, T. Salditt, "Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy", Nature Communications, Volume 9, Article number: 3641 (2018)

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, G. Donnert, "Three dimensional live-cell STED microscopy at increased depth using a water immersion objective", Review of Scientific Instruments (2018)

easy3D DyMIN STED volume imaging of Neurons with unprecedented resolution!

Confocal vs. DyMIN STED imaging:

Confocal and easy3D DyMIN STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to Abberior STAR 635P. Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany. Scale Bar, 2 µm.

DyMIN STED complements our existing list of intelligent STED illumination schemes, namely RESCue STED and MINFIELD STED. All three intelligent illumination schemes for STED dramatically reduce the light irradiation on your sample (DyMIN STED up to two orders of magnitude) and are exclusively available @ Abberior Instruments.

Explanation videos of DyMIN, RESCue and MINFIELD STED:

DyMIN STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017: 

J. Heine, M. Reuss, B. Harke, E. D'Este, S. J. Sahl and S. W. Hell, "Adaptive-illumination STED nanoscopy", PNAS (2017)

Nature review on DyMIN STED: G. Pacchioni, "Always look on the bright side of the fluorophore", Nature Review Materials (2017)