Same resolution but only 4 % of the typical light dose used – Do you think this is impossible? Please check out our images and movies:
RESCue 3D STED @ 775 nm. 3D reconstruction of a part of the nucleus recorded with RESCue 3D STED. Shown are Vero cells labelled with antibodies against lamin and secondary antibodies coupled to Abberior STAR 635P.
RESCue STED in living mammalian cells @ 595 nm. Note that more than 110 consecutive STED images have been recorded in the RESCue STED mode.
Shown are living Vero cells expressing ER-Citrine. Scale bar: 2 µm
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T. Staudt, A. Engler, E. Rittweger, B. Harke, J. Engelhardt, S. W. Hell, "Far-field optical nanoscopy with reduced number of state transition cycles", Opt. Express 19, 5644 – 5657 (2011).
L. Große, C. A. Wurm, C. Brüser, D. Neumann, D. C. Jans, S. Jakobs, "Bax assembles into large ring-like structures remodeling the mitochondrial outer membrane in apoptosis", EMBO J., (2016)