Same resolution but only 4 % of the typical light dose used – Do you think this is impossible?
Please check out our images and movies:
Note that RESCue STED with significantly reduced photobleaching enabled to image volume stacks using our pulsed 775nm and pulsed 595 STED laser.
Shown are 3 different views of the recorded superresolution 3-color volume data. Labelled structures: nuclear pore complex (blue, nup153, Oregon Green 488), vimentin (red, Abberior STAR635P) and mitochondria (green, tom20, Abberior STAR580).
RESCue 3D STED @ 775 nm. 3D reconstruction of a part of the nucleus recorded with RESCue 3D STED. Shown are Vero cells labelled with antibodies against lamin and secondary antibodies coupled to Abberior STAR 635P.
RESCue STED in living mammalian cells @ 595 nm. Note that more than 110 consecutive STED images have been recorded in the RESCue STED mode.
Shown are living Vero cells expressing ER-Citrine. Scale bar: 2 µm
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