RESCue STED

RESCue STED

Live-cell STED with REduction of State transition Cycles (RESCue STED)

Intelligent light dose management

 

  • RESCue avoids unnecessary excitation and de-excitation cycles and thus reduces photobleaching of any fluorescent marker
  • The reduction in excitation and de-excitation cycles is particularly beneficial for volume imaging with 3D STED as well as for time lapse STED imaging
  • RESCue STED applies excitation and STED light only at positions where fluorescent markers (i.e. the target structure) is present. It shuts off the lasers at positions where exposure would not make sense (i.e. where no markers are found)
  • The resolution of the image is maintained although the light dose is reduced down to 4 % compared to gated cw STED imaging and down to 20 % compared to pulsed STED imaging
  • RESCue STED is a highly photon-efficient way of superresolution imaging while conventional STED applies the same light dose to any pixel no matter if markers (i.e. a target) are present or not
  • RESCue can be applied with similar benefits also to confocal imaging

Same resolution but only 4 % of the typical light dose used – Do you think this is impossible? 

Please check out this short explanation video to learn more about our RESCue-STED:

      

 

Please check out our images and movies:

3-color easy3D RESCue STED imaging of mammalian cells

 

Note that RESCue STED with significantly reduced photobleaching enabled to image volume stacks using our pulsed 775nm and pulsed 595 STED laser.

 

Shown are 3 different views of the recorded superresolution 3-color volume data. Labelled structures: nuclear pore complex (blue, nup153, Oregon Green 488), vimentin (red, Abberior STAR635P) and mitochondria (green, tom20, Abberior STAR580).

Basic idea of RESCue:

Videos

RESCue 3D STED @ 775 nm.  3D reconstruction of a part of the nucleus recorded with RESCue 3D STED. Shown are Vero cells labelled with antibodies against lamin and secondary antibodies coupled to Abberior STAR 635P.

RESCue STED in living mammalian cells @ 595 nm. Note that more than 110 consecutive STED images have been recorded in the RESCue STED mode. 
Shown are living Vero cells expressing ER-Citrine. Scale bar: 2 µm

Further Information