The only superresolution microscope with true resolution below 20 nm* - MINFIELD confines the field of view to the inner minimum of the STED-doughnut (i.e. imaging fields of e.g. 100 x 100 nm). The excess doughnut intensity, connected to photobleaching, are sidestepped to the scan field. The dramatically higher signal allows increased resolutions below 20 nm at superior fluorescence contrast.
MINFIELD STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017:
F. Göttfert, T. Pleiner, J. Heine*, V. Westphal, D. Görlich, S. J. Sahl, S. W. Hell, "Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent", PNAS 1621495114 (2017)
MINFIELD STED presented on human immunodeficiency virus type 1 (HIV-1). (A) Confocal (left) and MINFIELD STED (right) images with a field size of 160 nm. (B) Scheme of the immatured HIV-1 particle*. (C) Imaging examples.
*Images taken from J. Hanne et. al."Stimulated Emission Depletion Nanoscopy Reveals Time-Course of Human Immunodeficiency Virus Proteolytic Maturation" ACS Nano 10, 8215-8222, 2016.
3D MINFIELD STED presented on standing DNA nanoruler with a nominal seperation of 91 nm, xz-view. (A) Confocal (left) and MINFIELD STED (right) images with a field size of 180 nm x 300 nm. (B) Imaging examples.
2D MINFIELD STED presented on GP210 and clathrin with both a MINFIELD size of 200 nm. (A) Imaging examples of GP210 (B) Imaging examples of clathrin.