STED < 20 nm resolution - MINFIELD STED

Intelligent light dose management


Key Benefits of MINFIELD:

The only superresolution microscope with true resolution below 20 nm* - MINFIELD confines the field of view to the inner minimum of the STED-doughnut (i.e. imaging fields of e.g. 100 x 100 nm). The excess doughnut intensity, connected to photobleaching, are sidestepped to the scan field. The dramatically higher signal allows increased resolutions below 20 nm at superior fluorescence contrast.

*depending on the fluorescent marker used

MINFIELD STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017:

F. Göttfert, T. Pleiner, J. Heine*, V. Westphal, D. Görlich, S. J. Sahl, S. W. Hell, "Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent", PNAS 1621495114 (2017)

*Abberior Instruments GmbH


MINFIELD complements an existing list of intelligent STED illuminations schemes:

Imaging Results

MINFIELD STED presented on human immunodeficiency virus type 1 (HIV-1). (A) Confocal (left) and MINFIELD STED (right) images with a field size of 160 nm. (B) Scheme of the immatured HIV-1 particle*. (C) Imaging examples.
*Images taken from J. Hanne et. al."Stimulated Emission Depletion Nanoscopy Reveals Time-Course of Human Immunodeficiency Virus Proteolytic Maturation" ACS Nano 10, 8215-8222, 2016.

3D MINFIELD STED presented on standing DNA nanoruler with a nominal seperation of 91 nm, xz-view. (A) Confocal (left) and MINFIELD STED (right) images with a field size of 180 nm x 300 nm. (B) Imaging examples.

2D MINFIELD STED presented on GP210 and clathrin with both a MINFIELD size of 200 nm. (A) Imaging examples of GP210 (B) Imaging examples of clathrin.