Abberior Instruments is proud to announce its new easy3D STED module which uses a completely novel approach to 3D STED microscopy. At its core, a programmable spatial light modulator (SLM) is used to create the phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations.
The SLM allows a revolutionary design with only one STED beam instead of two separate STED beams for lateral (xy) and axial (z) resolution enhancement, respectively. The resolution enhancement can be tuned between pure xy and mainly z enhancement. The single-beam design obsoletes any beam recombination and alignment resulting in unrivalled system stability.
Aberrations do matter! This measurement impressively demonstrates the effect of a refractive index mismatch. When an oil immersion lens is used to image a layer of fluorescent beads imaged through 13 μm of water, the image suffers from a significant loss in fluorescence intensity. This results in unusable images, both for confocal and for STED imaging (left).
In contrast, when a water immersion lens is used, both layers of beads are imaged with the same quality (right). The Abberior Instruments easy3D design allows you to change the objective lens on the fly and to use the most suitable lens in STED mode also!
H. Batoulis, T. H. Schmidt, P. Weber, J.-G. Schloetel, C. Kandt, T. Lang, "Concentration Dependent Ion-Protein Interaction Patterns Underlying Oligomerization Behaviours", Scientific Reports Apr. (2016)
B. Harke, C. K. Ullal, J. Keller, S. W. Hell, "Three-Dimensional Nanoscopy of Colloidal Crystals", Nano Lett. 8, 1309 (2008)
D. Wildanger, R. Medda, L. Kastrup, S. W. Hell, "A compact STED microscope providing 3D nanoscale resolution", J. Microsc. 236, 35 (2009)
B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, "Resolution scaling in STED microscopy", Opt. Express 16, 4154-4162 (2008)