easy3D STED

  • Spatial Light Modulator (SLM) technology
  • Resolution tunable between xy and z
  • Single STED beam
  • Enables the use of different objective lenses
  • Resolution typically 75 × 75 × 75 nm
  • Engineered for maximum stability
  • Correction of optical aberrations

The Abberior Instruments easy3D STED module uses a programmable spatial light modulator (SLM) to create phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations.

 

Easy3D STED anables to acquire 3D STED volumes deep in tissue

Adaptive optics easy3D STED enables deep imaging of cleared adult kidney samples 
Comparison of a XZ-slice deep into a renal corpuscle using conventional 3D STED imaging and an easy3D STED imaging. easy3D allowed imaging up to 80 µm into cleared rat kidney tissue using an oil objective. Without adaptive optics, the mismatch between immersion oil and sample leads to complete signal loss.

Labels: Nephrin (red, Abberior STAR635P) and Podocin (green, AlexaFluor594). Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

>> click Image for more details

 

Surface Rendering of 2-color easy3D STED volume of a cleared adult kidney sample of a rat

Shown is a reconstruction of an easy3D STED volume stack of a renal corpuscle showing Nephrin (red, Abberior STAR635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

 

Movie through a 2-color easy3D RESCue STED volume stack of a cleared adult kidney sample of a rat

Shown is a movie of an easy3D RESCue STED volume stack of a renal corpuscle showing Nephrin (red, Abberior STAR635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

2-color 2D STED image of a cleared adult kidney sample of a rat

Shown is an image of a renal corpuscle showing Nephrin (red, Abberior STAR635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

 

The SLM allows a revolutionary design with only one STED beam instead of two separate STED beams for lateral (xy) and axial (z) resolution enhancement, respectively. The resolution enhancement can be tuned between pure xy and mainly z enhancement. The single-beam design obsoletes any beam recombination and alignment resulting in unrivalled system stability.

Freely Choose Your Objective Lens for 3D STED Imaging

Freely Choose Your Objective Lens for 3D STED Imaging

Aberrations do matter! This measurement impressively demonstrates the effect of a refractive index mismatch. When an oil immersion lens is used to image a layer of fluorescent beads imaged through 13 μm of water, the image suffers from a significant loss in fluorescence intensity. This results in unusable images, both for confocal and for STED imaging (left).

In contrast, when a water immersion lens is used, both layers of beads are imaged with the same quality (right). The Abberior Instruments easy3D design allows you to change the objective lens on the fly and to use the most suitable lens in STED mode also!

Key Benefits

  • Tune the resolution in xy and z to fit your imaging problem
  • Use any combination of 2D or 3D STED phase plates or any user-defined phase modulation
  • Aberration correction: keep the STED PSF in shape even deep inside your sample (> 20 μm)
  • Switch between objective lenses on the fly: oil immersion, water immersion, silicone oil immersion...
  • Resolution easy3D: 90 × 90 × 90 nm minimum, 75 × 75 × 75 nm typical
  • Resolution 2D: better than 30 × 30 nm
  • Any Abberior Instruments Expert Line STED system can be upgraded with the easy3D STED module

Videos

Further Information