The only live-cell superresolution microscope with resolutions down to 25 nm* - DyMIN STED dramatically reduces the light irradiation on your sample (up to two orders of magnitude) and features the lowest light dose of all superresolution methods.
Resolution truly down to 25 nm - As demonstrated by separating two fluorescent point-structures being 30 nm apart.
Volume / time-lapse imaging with easy3D STED resolution - DyMIN STED substancially reduces photobleaching and enables long term measurements over volumes or over dozens and dozens of frames.
*only depending on the sample
DyMIN STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017:
Inspired by our unique RESCue concept DyMIN STED further reduces sample areas where maximum STED power is applied. This reduces the overall bleaching and enables to increase the reachable optical resolution with STED microscopy.
A second conceptual ingredient of DyMIN STED is MINFIELD STED, a co-development between Stefan Hell and coworkers and Abberior Instruments.
Please check out this short explanation video to learn more about our DyMIN-STED:
DyMIN STED complements an existing list of intelligent STED illumination schemes:
Confocal and easy3D DyMIN STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to Star635P. Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany. Scale Bar, 2 µm.
Easy3D DyMIN STED significantly reduces bleaching and thus enables the acquisition of a volume stack with an isotropic resolution of ~85 nm. Primary hippocampal neurons (22 days in vitro), betaII spectrin. Dye: Abberior STAR 635P, Excitation: 635 nm, STED: 775 nm. Sample with courtesy from Elisa D’Este (MPIbpc). Scale bars 200 nm.
DyMIN STED in living fibroplast cell. Note that 117 consecutive STED images have been recorded without fluorescence signal loss. Label: SiR-Tubulin.