Confocal RESCue

RESCue mode is Abberior Instruments’ unique & patent protected method for reducing the amount of photons sent into the sample while keeping image quality and resolution uncompromised.

Great news: it is not limited to STED imaging! For confocal microscopy, too, RESCue significantly reduces the light dose by up to 90% and more – again without losing any of the advantages of confocal imaging: speed, confocal resolution, z-sectioning ability, etc., are unchanged, while signal-to-noise, photobleaching behaviour, and live-cell survivability are significantly enhanced.

Check how we do live-cell confocal time-lapse imaging in confocal RESCue mode in an overnight measurement campaign with 14 hours of total imaging time. The cumulative light dose reduction was between 60% and 81% depending on the structure imaged. 

Don’t miss the ideal live-cell imaging conditions for your confocal experiments!

 

  • RESCue avoids unnecessary excitation cycles and thus reduces photobleaching of any fluorescent marker
  • The reduction in excitation cycles is particularly beneficial for volume imaging as well as for time-lapse imaging
  • RESCue improves live-cell compatibility by significantly reducing the light dose needed for confocal imaging – typically you realize between 60% and 90% total light dose reduction (depending on the structure imaged)
  • RESCue does not compromize the advantages of confocal imaging
  • RESCue is set up in a very easy way using the Abberior Instruments easyCommander 

Vero cells immunostained for the mitochondrial protein (PDH) and a peroxisomal membrane protein (PMP) using Cy3 and Cy5 conjugates. Z-stacks were recorded using standard confocal microscopy resulting in strong photobleaching after five consecutive stacks. Using confocal RESCue reduced photobleaching markedly and maintained image brightness after five consecutive Z-stacks.

 


 

Live fibroblasts were stained for tubulin using SiR-tubulin (silicone-containing rhodamine, Spirochrome) and mitochondria (MitoTracker Red CM-H2XRos, ThermoFisher) and imaged in HDMEM-GFP2 (Evrogen).

During 12 hours of live cell imaging, confocal RESCue allowed for a 82% reduction of laser irradiation for MitoTracker and a 50% reduction for SiR-tubulin.

Please note that overnight imaging with confocal RESCue was facilitated by the Abberior Instruments autofocus module (for confocal and STED).

Live fibroblasts were stained for tubulin using SiR-tubulin (silicone-containing rhodamine, Spirochrome) and mitochondria (MitoTracker Red CM-H2XRos, ThermoFisher) and imaged in in HDMEM (ThermoFisher).

During 14 hours of live cell imaging, confocal RESCue allowed for a 63% reduction of laser irradiation for MitoTracker, and a 53% reduction for SiR-tubulin.

Please note that overnight imaging with confocal RESCue was facilitated by the Abberior Instruments autofocus module (for confocal and STED).