Confocal Configurations

Confocal Configurations

Available confocal excitation lines:

  • 405nm (cw)/ 440nm (cw, pulsed)/ 470nm (cw, pulsed)/ 485nm (cw, pulsed)/ 518nm (cw, pulsed)/ 561nm (cw, pulsed)/ 594nm (cw, pulsed)/ 640nm (cw, pulsed); other wavelengths on request

 

Confocal scanner

  • Patented Abberior Instruments QUAD scanner with 4 galvo mirrors

 

Objectives

  • Any available objective for the respective microscope body

 

Detection

  • Detectors: new-generation APD (avalanche photo diode): high dynamic range (maximum 30 million counts per second; dead time <32ns; highest detection efficiency among all single-molecule detectors with 62% detection efficiency in the red fluorescence regime; single-molecule detection ability); other detectors on request (e.g. GaAsP PMT, hybrid detector)
  • Multi-channel (up to 4 detectors simultaneously)
  • Widefield detection
  • PSF detection

 

Confocality

  • Variable and motorized pinhole

 

Gating

  • Software based detector gating (e.g. gating of autofluorescence background signal)
  • Multiple gates per detector possible

 

Scanning modes

  • Line
  • Pixel
  • Pulse sequential

 

Further imaging modes

  • FLIM
  • FRET
  • FRAP
  • Transmitted light detection (TL)
  • DIC, phase contrast detection
  • Additional hardware can easily be integrated into the microscope. Additional digital and analog INS and OUTS are fully accessible and controllable via Imspector
  • Imaging mode with a non-confocal PMT detector to image the point-spread function (PSF) of all lasers in the sample plain
  • Remote control of the microscope via a Python or MATLAB script. Any parameter and setting within the Imspector software can be controlled via Python or MATLAB

Videos

Surface rendering of a confocal z-Stack of a pollen grain. Measured @ Expert Line Microscope on a Olympus IX83