775 STED
The 775 STED module enables STED nanoscopy at unprecedented resolutions with dyes in the far red spectrum.
- Pulsed STED laser @ 775nm featuring two superresolution STED channels
- Resolution up to 20 nm; typical resolution < 30 nm
- Two pulsed excitation laser sources @561nm and @640 nm, others on request
Impressions
Further Information
- Specs
- Pulsed STED laser @ 775nm featuring two superresolution STED channels
- Resolution up to 20 nm; typical resolution < 30 nm
- Two pulsed excitation laser sources @594nm and @640 nm. 594 nm is a proprietary Abberior Instruments pulsed excitation laser
- Change between objectives (water/ oil/ glycerol immersion)
- IX83 Olympus microscope or similar with a 4-color LED illumination source and a monochrome widefield camera
- Abberior QUAD scanner: novel scanner design with 4 galvo mirrors provides 4 degrees of freedom for independent position and angle settings; line frequency up to 2 kHz
- Abberior Patchpanel: electronic connectors (AO, AI, DIOs) for attaching custom external devices (e.g. laser sources etc.)
- Suitable for superresolution imaging with fluorescent proteins, e.g. mCherry as well as imaging with organic dyes, e.g. Abberior STAR 600 or STAR 635P
- Up to 8 time gating channels with <80 ps time resolution
- Up to 4 pulse-timing channels with <80 ps time resolution
- Multi-color acquisition method via interleave mode
(i) pulse interleaved and/or
(ii) pixel interleaved and/or
(iii) line-interleaved- Data acquisition system and software for image acquisition for different imaging modes (including 2-color STED mode)
- Software for data analysis (e.g. deconvolution algorithms etc.)
- Combinations
- Downloads
- References
J. Hanne, F. Goettfert, J. Schimer, M. Anders-Oesswein, J. Konvalinka, J. Engelhardt, B. Mueller, S. W. Hell, H.-G. Kraeusslich "Stimulated Emission Depletion Nanoscopy Reveals Time-Course of Human Immunodeficiency Virus Proteolytic Maturation", ACS Nano (2016)
O. Steshenko, D. M. Andrade, A. Honigmann, V. Mueller, F. Schneider, E. Sezgin, S. W. Hell, M. Simons, C. Eggeling, "Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy", Biophysical Journal 110, 2441–2450, Jun. (2016)
H. Batoulis, T. H. Schmidt, P. Weber, J.-G. Schloetel, C. Kandt, T. Lang, "Concentration Dependent Ion-Protein Interaction Patterns Underlying Oligomerization Behaviours", Scientific Reports, Apr. (2016)
K. Kehrein, R. Schilling, B. V. Möller-Hergt, C. A. Wurm, S. Jakobs, T. Lamkemeyer, T. Langer, M. Ott, "Organization of Mitochondrial Gene Expression in Two Distinct Ribosome-Containing Assemblies", Cell Rep., Feb. 12, pii: S2211-1247(15)00025-X (2015)
M. P. Clausen, S. Galiani, J. Bernardino de la Serna, M. Fritsche, J. Chojnacki, K. Gehmlich, B. C. Lagerholm, C. Eggeling,"Pathways to optical STED microscopy", Nanobioimaging 1-12, (2013)
G. Vicidomini, G. Moneron, K. Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S. W. Hell, "Sharper low-power STED nanoscopy by time gating", Nature Meth. 8, 571 - 573. (2011)
B. Harke, C. K. Ullal, J. Keller, S. W. Hell, "Three-Dimensional Nanoscopy of Colloidal Crystals", Nano Lett. 8, 1309 (2008)
L. Große, C. A. Wurm, C. Brüser, D. Neumann, D. C. Jans, S. Jakobs, "Bax assembles into large ring-like structures remodeling the mitochondrial outer membrane in apoptosis", EMBO J., (2016)
K. Kolmakov, C. A. Wurm, D. N. H. Meineke, F. Göttfert, V. P. Boyarskiy, V. N. Belov, S. W. Hell, "Polar Red-Emitting Rhodamine Dyes with Reactive Groups: Synthesis, Photophysical Properties, and Two-Color STED Nanoscopy Applications", Chem. Eur. J. 20, 146 - 157 (2014)
F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, S. W. Hell, "Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution", Biophysical Journal Volume 105, L01–L03 (2013)
C. A. Wurm, K. Kolmakov, F. Göttfert, H. Ta, M. Bossi, H. Schill, S. Berning, S. Jakobs, G. Donnert, V. N. Belov, S. W. Hell, "Novel red fluorophores with superior performance in STED microscopy", Optical Nanoscopy 1, 1 – 7 (2012)
K. Kolmakov, C. A. Wurm, R. Hennig, E. Rapp, S. Jakobs, V. N. Belov, S. W. Hell, "Red-Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy" Chemistry", A European Journal 18, 12986 - 12998 (2012)
B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, "Resolution scaling in STED microscopy", Opt. Express 16, 4154-4162 (2008)
B. Harke, J. V. Chacko, H. Haschke, C. Canale, A. Diaspro, " A novel nanoscopic tool by combining AFM with STED microscopy", Optical Nanoscopy 2012, 1:3
J. V. Chacko, B. Harke, C. Canale, A. Diaspro, "Cellular level nanomanipulation using atomic force microscope aided with superresolution imaging", J Biomed Opt. 2014; 19(10):105003.
J. V. Chacko, C. Canale, B. Harke, A. Diaspro, "Sub-diffraction nano manipulation using STED AFM", PLoS One. 2013 Jun 17;8(6):e66608
F. Göttfert, T. Pleiner, J. Heine, V. Westphal, D. Görlich, S. J. Sahl, S. W. Hell, "Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent", PNAS 1621495114 (2017)
- Labels
