EPYCON

EPYCON

EPYCON - Convert any epifluorescence microscope to confocal!

 

EPYCON readily converts any light microscope to a confocal with four fluorescence excitation lines:

  • 405nm (UV), 488nm, 561nm, 640nm
  • Installation within minutes 
  • Upgradable to Fluorescence Lifetime Imaging (FLIM)
  • Upgradable to STEDYCON: cutting-edge STED at 30 nm resolution
  • Highly affordable (please request a quote via email here)

 

Further details:

  • Fits every camera port
  • No warm-up time, ready within a minute
  • Easy-to-use, browser-based software (<15 minutes of training required)
  • Maintenance-free and low follow-up costs

Our compact line allows the following options:

Our STEDYCON & EPYCON work with all kinds of microscope bodies from every major vendor:

@ Leica DM2500
@ Leica DMI6000
@ Nikon NiE
@ Nikon Ti2
@ Nikon TiE
@ Olympus BX53
@ Olympus IX83
@ Zeiss Axio Imager
@ Zeiss Axio Observer with AFM
@ Zeiss Axio Observer Z1
@ Zeiss Axio Vert

Videos

From zero to STED in no time 

Follow along with the incredibly quick installation of a STEDYCON super resolution microscope, uncut. We go from opening the carton to the first super-resolution STED image in under three minutes. Same applies to our new EPYCON confocal microscope, which is upgradeable to a STEDYCON at any time.

Confocal image stack of cultured mammalian cell. Labelled are: Golgi Apparatus, green (GM130, Alexa Fluor 594); Actin, grey (Phalloidin-Oregon Green488); Peroxisomes, red (PMP70, Abberior STAR RED); DNA, blue (DAPI).

Confocal image stack of cultured mammalian cell. Labelled are: Golgi Apparatus, green (GM130, Alexa Fluor 594); Actin, grey (Phalloidin-Oregon Green488); Peroxisomes, red (PMP70, Abberior STAR RED); DNA, blue (DAPI).

Confocal image stack of cytoskeleton elements in cultured mammalian cells. Labelled are: Actin, green (Phalloidin-Oregon Green488); Tubulin, red (Abberior STAR RED); Vimetin, grey (Alexa Fluor 594); Nucleus, blue (DAPI).

Confocal image stack of cytoskeleton elements and mitochondria in cultured mammalian cell. Labelled are: Actin, red (Phalloidin-Oregon Green488); Tubulin, grey (Abberior STAR RED); Mitochondria, green (Tom20, Alexa Fluor 594); DNA, blue (DAPI).

Further Information

  • Specs
    • Excitation laser lines: 405nm (UV, cw), 488nm, 561nm, 640nm (pulsed)
    • 4 confocal imaging channels
    • Detection: APD1: 650-700nm; APD2: double bandpass 501-552nm and 578-628nm, benefit from unrivaled photon efficiency of APD detectors against any PMT detector variant; time-gated detection
    • FRET imaging mode
    • Upgradability to STEDYCON: 775nm pulsed STED laser upgradable; 2D STED resolution: <40nm, typically 30nm (depending on dyes and objectives used)
    • Upgradability to FLIM: get full FLIM functionality in all imaging channels through full compatibility with FLIM hardware from B+H or Picoquant
    • QUAD beam scanner: 90µm x 80µm field of view (with a 100x objective); 512x512 pixel up to 1.1 frames/s; line frequency up to 800Hz
    • Confocal pinhole: 1.1 Airy unit for a 100x objective
    • Software: browser-based control software operational on any modern PC, Mac or tablet: line interleaved confocal imaging in up to four imaging channels; implemented software features (not exhaustive): define volume (z-) stacks, acquisition queues and time series, auto-save function 
    • Dimensions: EPYCON scan-unit: 11cm x 20cm x 20cm; controller: 22cm x 55cm x 60cm; total weight head plus controller unit < 30kg
    • EPYCON is tested with all microscope stands and many objective lenses from every major vendor
    • Optionally: Optical table or antivibrational table, z-focusing piezo-drive, microscope stand and objective validated for EPYCON

     

    Please contact us for more details!